DSPP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71212-50, BS71212-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q9NZW4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF, IHC ICC

All Applications: WB: 1:500~1:2000
IHC: 1:100~1:500
IF: 1:100~1:500
ICC: 1:100~1:500

Background: This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DSPP polyclonal antibody detects endogenous levels of DSPP protein.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti-DSPP at 1/1000 dilution, Primary: Anti-DSPP at 1/1000 dilution, Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.

Alternative Name: DPP; DSP; DGI1; DMP3; DTDP2; DFNA39

Immunogen: KLH conjugated synthetic peptide derived from human Dentin sialoprotein: 101-220/1301

Conjugate: Unconjugated

Modification: Unmodified